Phosphorescence of pyridoxal.
نویسندگان
چکیده
The effect of pH on the relative distribution of pyridoxal structures in aqueous solution was examined by fluorescence spectroscopy. The hemiacetal structure, which is the predominant species at neutral pH, exhibits a symmetrical emission band at 378 rnp and is characterized by a quantum yield of 0.08. Luminescence studies at low temperature (77” K) have permitted the analysis of the phosphorescence properties of pyridoxal. The long lived emission of pyridoxal (phosphorescence lifetime, 1.1 set) originates in a triplet state (z -+ rr* transition) and shows a structureless phosphorescence band (X,, at 420 mp). An energy level diagram for pyridoxal is proposed, and the possible role of pyridoxal as a sensitizer in photobiological processes is discussed.
منابع مشابه
And Jorge
The effect of pH on the relative distribution of pyridoxal structures in aqueous solution was examined by fluorescence spectroscopy. The hemiacetal structure, which is the predominant species at neutral pH, exhibits a symmetrical emission band at 378 rnp and is characterized by a quantum yield of 0.08. Luminescence studies at low temperature (77” K) have permitted the analysis of the phosphores...
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 244 6 شماره
صفحات -
تاریخ انتشار 1969